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| González Pacheco, Blanca Estela. |
| El Tomato spotted wilt virus (TSWV, género Tospovirus, familia Bunyaviridae) se transmite por trips de manera persistente y se encuentra distribuido alrededor del mundo causando graves pérdidas económicas en diversos cultivos. El TSWV tiene genoma tripartita de ARN con las siguientes características principales: el segmento largo (L) es de polaridad negativa y codifica para la polimerasa RNA dependiente (RdRP); el segmento mediano (M) codifica las glicoproteínas Gn y Gc que forman parte de la cápside y NSm involucrada en el movimiento viral; y el segmento pequeño (S) codifica las nucleoproteínas NSs implicadas en la actividad supresora del silenciamiento genético. En México, el TSWV ha causado grandes pérdidas en cultivos ornamentales y hortícolas, de... |
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Palavras-chave: Tospovirus; TSWV; Marchitez manchada; Aislamientos; Tospoviruses; Spotted wilt; Isolates; Fitopatología; Doctorado. |
| Ano: 2014 |
URL: http://hdl.handle.net/10521/2271 |
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| Resende,Luciane V.; Maluf,Wilson R.; Figueira,Antônia dos R.; Resende,Juliano T.V.. |
| The commercial tomato, Lycopersicon esculentum, cultivars Ângela Gigante I-5100 and Santa Clara (tospovirus susceptible), the Stevens cultivar, lines and experimental hybrids (tospovirus resistant), were used: a) to evaluate two sources of resistance to tospoviruses in tomato (one from L. esculentum/Rey de Los Tempranos, and another one from L. peruvianum/Stevens); and b) to verify statistically significant correlation between virus concentration determined through DAS-ELISA and symptomatology. In the Ângela Gigante I-5100 and the Santa Clara cultivars, the correlation coefficient was highly significant and the symptom average level was high, showing that in susceptible cultivars the symptom evolution is related to the virus multiplication in the plant. As... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tospovirus; Breed lines; Viruses infection. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200014 |
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| BOARI,ALESSANDRA J.; MACIEL-ZAMBOLIM,EUNIZE; LAU,DOUGLAS D.; LIMA,GAUS S. A.; KITAJIMA,ELLIOT W.; BROMMONSCHENKEL,SÉRGIO S. H.; ZERBINI,F. MURILO. |
| The cubiu (Solanum sessiliflorum) fruit, originating in the Amazon basin, is commonly used in that region for food, medicine, and cosmetics. In an experimental culture of cubiu, in order to evaluate its adaptation to conditions in the Northern region of the state of Rio de Janeiro, it was observed plants with mosaic symptoms. A cubiu plant was collected and analyzed to identify the etiological agent. After mechanical passage through a local lesion host, a host range test was performed. The virus induced chlorotic local lesions in Chenopodium quinoa, necrotic local lesions in Gomphrena globosa, mosaic in S. sessiliflorum, leaf and stem necrosis in tomato (Lycopersicon esculentum) 'Rutgers', mosaic and leaf distortion in Datura stramonium and Physalis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tospovirus; Cubiu fruit; GRSV; Occurrence. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300002 |
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| Loos,Rodolfo A.; Silva,Derly José H. da; Fontes,Paulo Cezar R.; Picanço,Marcelo C.; Gontijo,Lessando M.; Silva,Ézio M. da; Semeão,Altair Arlindo. |
| Foram identificados e quantificados, no período de verão-outono, evidenciando o componente crítico e o fator-chave de perdas da cultura, mediante a utilização da tabela de vida das culturas. O trabalho foi realizado na Horta de Pesquisa da UFV, de janeiro a junho de 2001, sob delineamento de blocos casualizados, com dois tratamentos (cultivar Santa Clara I 5300 e híbrido Débora Plus) e cinco repetições. Durante o ciclo da cultura, foram avaliados o número de plantas mortas e as causas de morte, o número de flores e frutos/planta. Nas colheitas, os frutos sadios foram contados, pesados e classificados, sendo identificadas as causas de perda dos frutos danificados. Observou-se maior influência da perda de plantas (r=0,89) (P<0,01) sobre as perdas totais... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lycopersicon esculentum Mill.; Tabela de vida das culturas; Vira cabeça do tomateiro; Tospovirus; TSWV. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362004000200015 |
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| de Breuil,S; La Rossa,F.R; Giudici,A; Wulff,A; Bejerman,N; Giolitti,F; Lenardon,S. |
| Groundnut ringspot virus (GRSV), genus Tospovirus, is a thrips-transmitted virus infecting peanuts (Arachis hypogaea L.) in Córdoba province, Argentina. Fourteen viral isolates were recovered from Tospovirus-like symptomatic plants from different peanut fields. Viral isolates as GRSV were identified by serological and molecular tests. Nucleotide and derived amino acid sequence analyses of the nucleocapsid (N) gene indicated a high degree of identity between the GRSV peanut isolates, indicating that there is no molecular variability in the N gene of the GRSV that infects peanuts in the cropping area of Córdoba. In this study, we determined the presence of thrips species in the crop, which can potentially transmit the virus. Thrips were observed in all the... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Arachis hypogaea; Tospovirus; Molecular characterization; Frankliniella schultzei. |
| Ano: 2015 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2015000100007 |
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| Aguiar,Raimundo Wagner de Souza; Rodrigues,Adelmo; Portella,Augustus Caeser Franke; Lopes,Magnolia de Mendonça; Lima,Mirtes Freitas; Resende,Renato de Oliveira; Nagata,Tatsuya. |
| Watermelon (Citrullus lanatus) cultivated in almost all tropical and subtropical regions of the world, has its largest output in China, and then, according to FAO data, Turkey, Iran and Brazil, being one of the main crops cultivated in State of Tocantins, Brazil. In this work was investigated the occurrence and distribution of the watermelon viruses, totaling 752 samples taken in a stratified experimental design in four representative regions of production: Gurupi (150), Lagoa da Confusao (232), Formoso do Araguaia (265) and Porto Nacional (105). The sampling and collecting the leaves of plants with the presence of symptoms were performed once a week during the entire cultivation cycle. As a result, were observed by Dot-ELISA method, different types of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Watermelon; Citrullus lanatus; Potyvirus; Cucumovirus; Tospovirus; Serological assay. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200192 |
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| Colariccio,Addolorata; Eiras,Marcelo; Chaves,Alexandre L. R.; Harakava,Ricardo; Chagas,César M.. |
| In the regions of Campinas and Sumaré, São Paulo, Brazil, hidroponically grown crops of Lettuce (Lactuca sativa) cv. Verônica, which showed virus-like symptoms were examined by electron microscope, biological, serological and molecular tests. Pleomorphic, enveloped particles (80-100 nm in diameter) were always detected in these samples. Experimentally inoculated host plants, including lettuce, reacted with tospoviruses-induced symptoms. Some differences were observed in Gomphrena globosa, which reacted by showing local lesions and systemic mosaic. Two isolates of Tomato chlorotic spot virus (TCSV) were identified by DAS-ELISA and by RT-PCR. The sequencing and alignment of the RT-PCR coat protein amplified fragments have indicated a high degree of homology... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Tospovirus; Serology; RT-PCR; Sequencing. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000300012 |
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